The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation
Identifieur interne : 005253 ( Main/Exploration ); précédent : 005252; suivant : 005254The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation
Auteurs : Andrew Parkinson ; Stephen SafeSource :
- Journal of Pharmacy and Pharmacology [ 0022-3573 ] ; 1979-09.
Abstract
A comparison was made of various indices of the hepatic drug‐metabolizing apparatus associated with the postmitochondrial supernatant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone‐pretreated and 3‐methylcholanthrene‐pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P‐450 and by the activity of NADPH‐cytochrome c reductase, 4‐dimethylaminoantipyrine N‐demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes.
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DOI: 10.1111/j.2042-7158.1979.tb13550.x
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